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Splice Site Prediction by Neural Network

PLEASE NOTE: This server runs the NNSPLICE 0.9 version (January 1997) of the splice site predictor.

Prediction Options

Organism:

Search for 5', 3' or both splice sites?

Include reverse strand?

Minimum score for 5' splice site (between 0 and 1):

Minimum score for 3' splice site (between 0 and 1):

Sequences

Cut and paste your sequence(s) here:
Use single-letter nucleotides (A, C, G, T). You can include multiple sequences if each has a FASTA title line starting with >.

Please be patient—splice site prediction may take a while.

Training set

Our training and test sets of human and Drosophila melanogaster splice sites are available to the community for testing splice site predictors. They can be obtained from our collection of representative, standardized data sets of human and D. melanogaster genes.

Need promoter predictions?

Try the companion Neural Network Promoter Prediction tool for identifying transcription start sites.

Publication

Reese MG, Eeckman FH, Kulp D, Haussler D, 1997. “Improved Splice Site Detection in Genie”. J Comp Biol 4(3), 311-23.

Splice site prediction code by Martin G. Reese; web interface by Nomi Harris.