BarcodeMetric

Metrics

Category Metrics


Overview

Metrics produced by the ExtractIlluminaBarcodes program that is used to parse data in the basecalls directory and determine to which barcode each read should be assigned.

This table summarizes the values that are specific to this metric.

Metric Summary
BARCODE
The barcode (from the set of expected barcodes) for which the following metrics apply. Note that the "symbolic" barcode of NNNNNN is used to report metrics for all reads that do not match a barcode.
BARCODE_WITHOUT_DELIMITER
BARCODE_NAME
The barcode name.
LIBRARY_NAME
The name of the library
READS
The total number of reads matching the barcode.
PF_READS
The number of PF reads matching this barcode (always less than or equal to READS).
PERFECT_MATCHES
The number of all reads matching this barcode that matched with 0 errors or no-calls.
PF_PERFECT_MATCHES
The number of PF reads matching this barcode that matched with 0 errors or no-calls.
ONE_MISMATCH_MATCHES
The number of all reads matching this barcode that matched with 1 error or no-call.
PF_ONE_MISMATCH_MATCHES
The number of PF reads matching this barcode that matched with 1 error or no-call.
PCT_MATCHES
The fraction of all reads in the lane that matched to this barcode.
RATIO_THIS_BARCODE_TO_BEST_BARCODE_PCT
The rate of all reads matching this barcode to all reads matching the most prevalent barcode. For the most prevalent barcode this will be 1, for all others it will be less than 1 (except for the possible exception of when there are more orphan reads than for any other barcode, in which case the value may be arbitrarily large). One over the lowest number in this column gives you the fold-difference in representation between barcodes.
PF_PCT_MATCHES
The fraction of PF reads in the lane that matched to this barcode.
PF_RATIO_THIS_BARCODE_TO_BEST_BARCODE_PCT
The rate of PF reads matching this barcode to PF reads matching the most prevalent barcode. For the most prevalent barcode this will be 1, for all others it will be less than 1 (except for the possible exception of when there are more orphan reads than for any other barcode, in which case the value may be arbitrarily large). One over the lowest number in this column gives you the fold-difference in representation of PF reads between barcodes.
PF_NORMALIZED_MATCHES
The "normalized" matches to each barcode. This is calculated as the number of pf reads matching this barcode over the sum of all pf reads matching any barcode (excluding orphans). If all barcodes are represented equally this will be 1.

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