TargetedPcrMetrics

Metrics

Category Metrics


Overview

Metrics class for the analysis of reads obtained from targeted pcr experiments e.g. the TruSeq Custom Amplicon (TSCA) kit (Illumina).

This table summarizes the values that are specific to this metric.

Metric Summary
CUSTOM_AMPLICON_SET
The name of the amplicon set used in this metrics collection run
AMPLICON_TERRITORY
The number of unique bases covered by the intervals of all amplicons in the amplicon set
ON_AMPLICON_BASES
The number of PF_BASES_ALIGNED that mapped to an amplified region of the genome.
NEAR_AMPLICON_BASES
The number of PF_BASES_ALIGNED that mapped to within a fixed interval of an amplified region, but not on a baited region.
OFF_AMPLICON_BASES
The number of PF_BASES_ALIGNED that mapped neither on or near an amplicon.
PCT_AMPLIFIED_BASES
The fraction of PF_BASES_ALIGNED that mapped to or near an amplicon, (ON_AMPLICON_BASES + NEAR_AMPLICON_BASES)/PF_BASES_ALIGNED.
PCT_OFF_AMPLICON
The fraction of PF_BASES_ALIGNED that mapped neither onto or near an amplicon, OFF_AMPLICON_BASES/PF_BASES_ALIGNED
ON_AMPLICON_VS_SELECTED
The fraction of bases mapping to regions on or near amplicons, which mapped directly to but not near amplicons, ON_AMPLICON_BASES/(NEAR_AMPLICON_BASES + ON_AMPLICON_BASES)
MEAN_AMPLICON_COVERAGE
The mean read coverage of all amplicon regions in the experiment.
FOLD_ENRICHMENT
The fold by which the amplicon region has been amplified above genomic background.

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