WgsMetrics

Metrics

Category Metrics


Overview

Metrics for evaluating the performance of whole genome sequencing experiments.

This table summarizes the values that are specific to this metric.

Metric Summary
GENOME_TERRITORY
The number of non-N bases in the genome reference over which coverage will be evaluated.
MEAN_COVERAGE
The mean coverage in bases of the genome territory, after all filters are applied.
SD_COVERAGE
The standard deviation of coverage of the genome after all filters are applied.
MEDIAN_COVERAGE
The median coverage in bases of the genome territory, after all filters are applied.
MAD_COVERAGE
The median absolute deviation of coverage of the genome after all filters are applied.
PCT_EXC_ADAPTER
The fraction of aligned bases that were filtered out because they were in reads with mapping quality 0 and the looked like adapter reads.
PCT_EXC_MAPQ
The fraction of aligned bases that were filtered out because they were in reads with low mapping quality (lower than MIN_MAPPING_QUALITY).
PCT_EXC_DUPE
The fraction of aligned bases that were filtered out because they were in reads marked as duplicates.
PCT_EXC_UNPAIRED
The fraction of aligned bases that were filtered out because they were in reads without a mapped mate pair.
PCT_EXC_BASEQ
The fraction of aligned bases that were filtered out because they were of low base quality (lower than MIN_BASE_QUALITY).
PCT_EXC_OVERLAP
The fraction of aligned bases that were filtered out because they were the second observation from an insert with overlapping reads.
PCT_EXC_CAPPED
The fraction of aligned bases that were filtered out because they would have raised coverage above COVERAGE_CAP.
PCT_EXC_TOTAL
The total fraction of aligned bases excluded due to all filters.
PCT_1X
The fraction of bases that attained at least 1X sequence coverage in post-filtering bases.
PCT_5X
The fraction of bases that attained at least 5X sequence coverage in post-filtering bases.
PCT_10X
The fraction of bases that attained at least 10X sequence coverage in post-filtering bases.
PCT_15X
The fraction of bases that attained at least 15X sequence coverage in post-filtering bases.
PCT_20X
The fraction of bases that attained at least 20X sequence coverage in post-filtering bases.
PCT_25X
The fraction of bases that attained at least 25X sequence coverage in post-filtering bases.
PCT_30X
The fraction of bases that attained at least 30X sequence coverage in post-filtering bases.
PCT_40X
The fraction of bases that attained at least 40X sequence coverage in post-filtering bases.
PCT_50X
The fraction of bases that attained at least 50X sequence coverage in post-filtering bases.
PCT_60X
The fraction of bases that attained at least 60X sequence coverage in post-filtering bases.
PCT_70X
The fraction of bases that attained at least 70X sequence coverage in post-filtering bases.
PCT_80X
The fraction of bases that attained at least 80X sequence coverage in post-filtering bases.
PCT_90X
The fraction of bases that attained at least 90X sequence coverage in post-filtering bases.
PCT_100X
The fraction of bases that attained at least 100X sequence coverage in post-filtering bases.
FOLD_80_BASE_PENALTY
The fold over-coverage necessary to raise 80% of bases to the mean coverage level.
FOLD_90_BASE_PENALTY
The fold over-coverage necessary to raise 90% of bases to the mean coverage level.
FOLD_95_BASE_PENALTY
The fold over-coverage necessary to raise 95% of bases to the mean coverage level.
HET_SNP_SENSITIVITY
The theoretical HET SNP sensitivity.
HET_SNP_Q
The Phred Scaled Q Score of the theoretical HET SNP sensitivity.

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GATK version 4.6.2.0 built at Sun, 13 Apr 2025 13:21:43 -0400.